【摘要】 目的对轻症和重症H7N9禽流感病毒菌株的致病性进行分析,并探讨病毒分子特征与其致病差异间的关联性。方法通过建立三种模型即PBMC人体外培养及病毒感染,人肺组织体外培养及病毒感染及小鼠感染H7N9动物模型,分别对轻症和重症H7N9携带病毒感染者其分离株包括GD-6和GD-7,对两株病毒的感染性及病理情况进行研究分析。结果病毒对三种模型组织均能感染并进行有效复制但复制GD-7的能力高于GD-6;在小鼠肺组织内,其重症分离株GD-7病毒增殖力较轻症分离株GD-6要高且对于小鼠的致病力其重症分离株GD-7较轻症分离株GD-6强。结论GD-7作为重症分离菌株其复制能力及致病能力均较GD-6轻症分离株要强。更多还原

【Abstract】 Objective To analyze the pathogenicity of mild strain and severe strain H7N9 of avian influenza virus and to explore the association between the molecular characteristics of viruses and their pathogenic differences. Methods GD-6 and GD-7 were isolated from patients infected with mild or severe H7N9 virus. There models were used in this research, PBMC in vitro cultured and infected by virus, human lung tissue culture and virus infection and mouse infected with H7N9 animal model. Then the pathogenicity of the two strains of the H7N9 virus were analyzed and compared. Results In the three models the virus could infect tissue and reproduce, and GD-7 strain more effectively than GD-6 strain. In the lung tissue of mice, the degeneration of GD-7 virus with higher pathogenicity than that of GD-6 strain. Conclusion GD-7 as a severe isolated strain has stronger ability of replication and pathogenicity than GD-6 strain which isolated from mild H7N9.更多还原