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半胱胺脂质体对梅花鹿增重及血液生长抑素、生长激素含量的影响
The Effects of Liposome-Cysteamine on Body Weight Gain and the Somatostatin/Growth Hormone(GH) Content of Blood in Sika Deer(Cervus nippon Temminck)
【摘要】 选择日龄60天的梅花鹿120头,随机分为4组,每组30头,在4个组精饲料中添加半胱胺脂质体剂量分别为0(对照)、100、150、200mg/kg体重。至180日龄称重、静脉采血,并用放免法对血液样品中生长抑素(SS)和生长激素(GH)含量进行分析,用钼蓝法721分光光度计及凯氏定氮法测定饲料表观消化率。结果显示:(1)与对照组相比,CS100组净增重提高48.3%(P<0.05),其它剂量组增重无明显变化;(2)血液中SS的含量CS100、CS150、CS200组分别下降69.6%(P<0.05)、75.4%(P<0.025)和75.8%(P<0.01),GH水平只在CS100组有明显升高(P<0.05);(3)CS100对梅花鹿钙、磷、粗蛋白表观消化率影响的方差分析结果达到了显著的水平(P<0.05)(与对照组相)。(4)梅花鹿成活率提高了13.3%。
【Abstract】 Experiments carried out on120 sika deer at the age of 60 days,divided into 4 groups at random,feeding diets with 0(control group) ,100,150,200mg/kg liposome-cysteamine.The blood samples were weighed and collected at 180 days of age.The content of somatostatin(SS) /growth hormone(GH) was analyzed by radioimmunoassay method and the apparent digestibility was evaluated by spectrophotometer and the Kjeldahl method.The result showed that compared with control group,the weight of the group CS100 increased by 48.3%(P<0.05) ,while the others didn’t.The content of SS decreased in all experimental groups(CS100,CS150,CS200) by 9.6%(P<0.05) ,75.4%(P<0.025) and75.8%(P<0.01) ,respectively,and only CS100 group had a GH increase.The variance analysis result of effect of CS100 on the apparent digestibility of calcium,phosphorus and crude protein was remarkable(P<0.05) .The survival rate of sika deer increased by 13.3%.
【Key words】 Cysteamine liposome; spotted deer; somatostatin; growth hormone;
- 【文献出处】 黑龙江科学 ,Heilongjiang Science , 编辑部邮箱 ,2010年01期
- 【分类号】S825.5
- 【下载频次】20