【摘要】 采用稀释平板分离法获得67株真菌,测定其维生素E(VE)含量,以期从土壤中筛选出富含天然VE的真菌新资源。采用高效液相色谱法进行VE含量检测,其中有10株真菌能产生天然VE;通过形态与分子系统学手段对10株菌株进行鉴定,有5株菌株鉴定到种,分别属于马昆德拟青霉(Paecilomyces marquandii)、爪哇棒束孢(Isaria javanica)和淡紫紫孢菌(Purpureocillium lilacinum),2株菌株鉴定到属,均为戴氏霉属(Taifanglania);另外3株菌株序列未测出。对其中两株VE含量较高的菌株——马昆德拟青霉和爪哇棒束孢从碳源和氮源方面进行了初步的培养基优化,结果表明不同的碳源、氮源对VE含量均有一定影响。更多还原

【Abstract】 67 fungal strains were obtained by dilution plate;and their natural vitamin E content was determined by fluorescence spectrophotometry and HPLC to screen new resources of natural vitamin E producing fungi.10 strains were identified as vitamin E producing fungi.According to morphology and molecular systematics,5 strains were identified as Paecilomyces marquandii,Isaria javanica,Purpureocillium lilacinum;2 strains belonged to Taifanglania genus;and 3 strains was not able to be sequenced.The carbon and nitrogen sources of medium for two high vitamin E producing strains,P.marquandii and I.javanica,was optimized;and results showed that different nitrogen and carbon sources affected their vitamin E content to a certain degree.更多还原