【摘要】 本文研究了盐酸-乙醇等电点法脱除油茶籽多糖中蛋白质的方法,此法在盐酸-等电点法的基础上,添加一定量的乙醇溶液,使之更有利于蛋白质的沉淀脱除。通过单因素试验及正交试验,确定蛋白质脱除的最佳条件为:pH 3.4,乙醇添加量为13%,静止时间为3 d,可以使蛋白质脱除率达到92.68%,多糖保留率为61.78%。与传统的三氯乙酸法和Sevage法进行比较,此法更有效且更安全。更多还原

【Abstract】 This paper studied a new type of proteins removal method from camellia polysaccharides,hydrochloric acid-ethanol isoelectric point method.On the basis of hydrochloric acid isoelectric point method,the new method adding a certain amount of ethyl alcohol into suspension is more effective than the traditional methods to precipitate proteins.Through single factor experiments and orthogonal test,the optimum condition was found as follows: pH 3.4,ethyl alcohol adding 13% and standing time 3 days,under which protein removal rate and polysaccharides yield reached 92.68% and 61.78,respectively.Compared with the traditional method of trichloroacetic acid(TDA) and Sevage,it was more effective and more safe.更多还原