【摘要】 视觉电生理检测已成为眼科临床常用的功能性检查方法之一。小动物视觉电生理是研究小动物视觉系统功能的重要工具，在神经科学和药物研发领域具有广泛应用。指南旨在提供一份小动物视觉电生理规范操作指南，以确保测试的准确性和可重复性。文章重点介绍了不同类型的小动物视觉电生理测试，包括视网膜电反应图(ERG)和视觉诱发电位(VEP),以及它们在不同疾病模型中的应用。随后，对实验动物的选择和准备工作进行了详细描述，包括动物种类、麻醉方法和测试环境的要求。在操作步骤方面，指南强调了正确的电极放置、刺激参数的选择以及信号采集和处理的关键步骤。针对不同动物模型，提供了相应的操作建议，并系统阐述了常见问题的解决方案。除了基本操作，指南还关注了测试结果的解释和报告，对不同类型的电生理波形进行了解释。小动物视觉电生理操作规范为研究人员提供了一个全面且详细的操作框架，以确保小动物视觉电生理测试的标准化和可靠性。通过遵循这些指南，研究人员能够更好地利用小动物视觉电生理技术，深入了解视觉系统的功能和异常。更多还原

【Abstract】 Visual electrophysiology measurements have become a routine method of functional examination in ophthalmology. Small animal visual electrophysiology is an important tool for exploring the functionality of the visual system in small animals, finding widespread applications in neuroscience and drug research and development. This guide aims to offer a standardized operational guide for the operation of visual electrophysiological norms in small animals to ensure the accuracy and repeatability of the test. The study emphasizes different types of visual electrophysiological tests, such as electroretinogram(ERG) and visual evoked potential(VEP), evoked in small animals, and their application in different disease models. Detailed descriptions are provided regarding the selection and preparation of experimental animals, including the requirements of animal species, anesthesia methods and test environment. In terms of operational procedures, this guide highlights the correct electrode placement, the selection of stimulus parameters, and the key steps for signal acquisition and processing. According to different animal models, the corresponding operation suggestions were provided, and the troubleshooting methods of common problems were introduced. Beyond fundamental operations, this guide also focuses on the interpretation and reporting of test results. It explains various types of electrophysiological waveforms. In summary, this operational specification for small animal visual electrophysiology provides a comprehensive and detailed framework for researchers to ensure the standardization and reliability of tests. By following these guidelines, researchers can effectively utilize small animal visual electrophysiology techniques to gain insight into the function and abnormalities of the visual system.更多还原