【摘要】 目的探索microRNA在四氯化碳(CCl4)诱导的大鼠纤维化肝组织中的差异表达及其意义。方法采用microRNA高通量测序分析技术检测对照组(n=10)与模型组(n=10)肝脏组织microRNA表达,对差异microRNA进行靶基因预测,对靶基因进行基因本(GO)分析、pathway分析。结果对照组与模型组间筛选出37个差异microRNA,上调29个,下调8个;从microRNA-基因网络图中发现关键上调为miR-184、miR-10b-5p、miR-199a-3p等;关键下调为miR-200b-3p、miR-199a-5p、miR-125b-5p等。结论模型组microRNA表达谱变化明显,肝纤维化的形成与microRNA调控细胞增殖、凋亡、细胞周期等有关。更多还原

【Abstract】 Objective To explore the differences of expression profiles and significance of the microRNA in liver tissue of rat induced by carbon tetrachloride(CCl4).Methods The microRNA differential expression analysis between normal group(n=10)and model group(n=10)was conducted on high-throughput methods.Gene ontology enrichment analysis and kyoto encyclopedia of genes and genomes pathway analysis of the targets of miRNA were carried out in this study.Results Some differentally expressed microRNA with 29up-regulated and 8down-regulated were identified in our study.Through evaluaing the value of microRNA in the network,we find that the key microRNA of rising are miR-184,miR-10b-5p,miR-199a-3p,and the key microRNA of cutting are miR-200b-3p,miR-199a-5p,miR-125b-5p and so on.Conclusion The microRNA expression level in the model group has a significant change.Therefore,the courses of liver fibrosis is related to some process which microRNA control the regulation of cell proliferation,cell apoptosis,cell cycle,and so on.更多还原