【摘要】 通过紫外光谱法、荧光光谱法、核磁滴定以及分子对接模拟等实验方法探究车前草中黄酮类化合物与黄嘌呤氧化酶（XO）之间的抑制作用。结果表明：5种黄酮类化合物均表现出较好的酶抑制效果；异泽兰黄素是抑制效果最好的化合物，其半抑制浓度(IC₅₀)为0.72μmol·L^-1;黄酮化合物B环在酶抑制方面起重要作用，B环R₁和R₂位置上基团相同且疏水性较大时更有利于目标化合物与酶疏水性空腔的结合；抑制剂通过氢键和疏水作用力与酶结合；异泽兰黄素通过氢键、范德华力、π-σ键和疏水作用力等与酶结合。从而证明车前草可以作为健康无毒的黄嘌呤氧化酶抑制剂，缓解高尿酸血症以及痛风。更多还原

【Abstract】 The inhibition of the interaction between flavonoids and xanthine oxidase（XO） in plantain was studied by ultraviolet spectroscopy, fluorescence spectroscopy, ¹H NMR and molecular docking.The results showed that the five flavonoids showed they can inhibit enzyme activity.The half inhibitory concentration(IC₅₀) of isoparaflain that the best inhibitory compound was 0.72 μmol·L^-1. The B ring of flavonoid play an important role in the enzyme inhibition, R₁ and R₂ of B ring at the same position, that higher hydrophobicity would be very important and helpful for enhancing the combination between target compound and the hydrophobic domain of（XO）.Inhibitors bind to enzymes through hydrogen and bondshydrophobic forces.Eupatilin bind to enzymes through hydrogen bonds, van der Waals forces, π-σ bonds, hydrophobic forces and so on.Thus it proved that plantain could be used as a nontoxic xanthine oxidase inhibitor to relieve hyperuricemia and gout.更多还原