【摘要】 建立同时测定香茅油中香叶醇、柠檬醛、香茅醛、月桂烯的反相高效液相色潽法(RPHPLC)。色谱柱为Agilent Eclipse XDB-C18(4.6mm×250mm,5μm),流动相为乙腈-水(80∶20,v/v),流速为1.0 m L/min,检测波长为210 nm,柱温为室温,进样量20μL。在此色谱条件下,4组分获得良好的分离且各组分的质量浓度与峰面积呈良好线性关系,相关系数R2为0.9977~0.9998。样品中各组分的加标回收率在96.4%~99.2%之间,RSD≤2.0%。所建立的HPLC法操作简便,有良好的稳定性及重现性,适用于香茅油中主要成分含量的测定。更多还原

【Abstract】 A method of determining geraniol,citral,citronellal and myrcene in citronella oil simultaneously by reversed- phase high performance liquid chromatography( RP- HPLC) was established. Chromatography column was Agilent Eclipse XDB- C18( 4. 6 250 mm,5 m),mobile phase was acetonitrile- water( 80 ∶ 20,v / v),flow rate was 1. 0 m L / min,detection wavelength was 210 nm,column temperature was room temperature,and injection volume was 20μL. Under these chromatographic conditions,four analytes were well separated,and a good linear relationship between peak area and concentration was observed for each component. The correlation coefficient R2 was varied from0. 9977 to 0. 9998. The average spike recoveries of geraniol,citral and myrcene was form 96. 4% to 99. 2%,with RSD≤2. 0%. This method was simple and had good stability and reproducibility. It was suitable for the determination of these compounds in citronella oil.更多还原