【摘要】 目的:为了了解天王补心丸全方的安神作用机制以及桔梗对天王补心丸催眠作用的影响。方法:建立小鼠戊巴比妥钠协同睡眠模型,考察天王补心丸全方及全方缺桔梗对小鼠睡眠的影响;建立少寐大鼠的模型,以睡眠觉醒相关神经递质5-HT,NA及DA为分析指标,通过HPLC-ECD检测,考察桔梗配伍与否对脑内神经递质及其代谢产物的影响。结果:天王补心丸全方能够显著地性增加阈下剂量戊巴比妥钠协同睡眠的入睡率(P<0.05),而天王补心丸全方缺桔梗组只有使睡眠率增加的趋势,而无统计学差异;给药后全方组、全方缺桔梗组均显著性升高了大鼠中缝核单胺类递质5-TH及5-HIAA水平(P<0.05),且全方缺桔梗组与全方组相比有显著性差异;而在海马区与纹状体区只有全方组显著性升高促睡眠递质5-TH水平(P<0.05),且与全方缺桔梗组相比有显著性差异(P<0.05);全方及全方缺桔梗组均显著降低大鼠下丘脑DA水平(P<0.05):全方及全方缺桔梗组均显著地降低纹状体内DA代谢产物DOPAC水平(P<0.05)。结论:天王补心丸全方催眠作用机制在于促进了各脑区及核团内5-HT释放,而桔梗将有可能为调节的关键点。更多还原

【Abstract】 Objective:To understand the action mechanism of Tianwang Buxin decoction that is the whole prescription included all drugs from Tianwang Buxin honeyed pill and Tianwang Buxin without radix platycodi decoction on the nerves-calming and hyposomnia-curing.Method:The influence of Tianwang Buxin decoction and Tianwang Buxin without radix platycodi decoction on somnus utilizing the mice’ s somnus in coordination with Pentobarbital sodium was observed.Investigation whether the compatibility of radix platycodi affect the concentration of brain neurotransmitter,5-HT,NA and DA,correlated sleep-awareness by HPLC-ECD detection was carried out after rats’ hyposomnia model were founded.Result:The falling asleep rates of mice given subthreshold dose raised (P<0.05),remarkably because of Tianwang Buxin decoction.But there is significant difference with Tianwang Buxin lack of radix platycodi decoction despite the heightening tendency.All groups were discovered that the level of 5-HT and 5-HIAA,the monoamine transmitter,heighten obviously after the whole prescription and the prescription without radix platycodi were administered in nuclei raphae medullae oblongatae (P<0.05),but it is only the whole prescription group that emerged same phenomenon in the Ammon’s horn and striatum area.Furthermore significant difference exist as comparing Tianwang Buxin whole prescription decoction with Tianwang Buxin without radix platycodi decoction.The level of another monoamine transmitter DA stepped up notably in the whole prescription and the prescription without radix platycodi groups following administration in corpora striata (P<0.05).Conclusion:The mechanism of hypnosis action lie in enhancement of releasing 5-HT in encephalic regions for the Tianwang Buxin whole prescription decoction,but it’s possible that radix platycodi may be the key point that adjusts the additional quantity.更多还原