【摘要】 以通过生理休眠的紫皮大蒜Z-006-10鳞茎茎尖为试材,利用固体培养基中添加甲基胺草磷的离体诱导方法,研究甲基胺草磷不同处理浓度和时间对紫皮大蒜体细胞染色体的诱导效果,并利用形态观察、根尖细胞染色体计数及流式细胞仪分析等方法对得到的再生植株进行倍性鉴定。试验结果表明:10μmol.L-1甲基胺草磷处理6d,四倍体诱导率最高,达32.87%。形态观察结果表明,得到的四倍体植株多倍体形态特征明显,与二倍体植株差异明显。根尖染色体压片观察表明,四倍体染色体数为2n=4x=32,对照为2n=2x=16。流式细胞仪倍性分析表明,四倍体体细胞DNA的相对含量是二倍体的2倍,其结果与染色体计数法鉴定结果一致。更多还原

【Abstract】 Taking bulb shoot tips of purple garlic(Allium sativum L.)Z-006-10 passed physiological dormancy as experimental material,using in vitro induction method by adding Amiprophos-methyl into solid culture,studied the induction effect of Amiprophos-methyl on chromosome of somatic cell at different concentration and with different treated durations.The ploidy level was identified by morphological trait,chromosome number and flow cytometry techniques.The results indicated that the treatment with 10 μmol·L-1 Amiprophos-methyl for 6 d,the induction rate of tetraploid reached the highest-32.87%.The morphological trait showed that the tetraploid plants showed obviously morphological characteristics and were remarkably differed from diploid plants.The chromosome observation of root tips showed that the choromosome number of tetraploids was 2n=4x=32,while the control was 2n=2x=16.The ploidy analysis by flow cytometry verified that the relative DNA content of tetraploid somatic cell was twice the diploids,which was accorded with that of chromosome analysis.更多还原