【摘要】 目的:建立双柏散(大黄,侧柏叶,关黄柏,泽兰和薄荷)HPLC指纹图谱分析方法,拟定其指纹特征图谱指标成分群。方法:采用HPLC法测定,色谱柱为ZORBAX Eclipse XDB-C18;流动相为乙腈-0.1%磷酸溶液(梯度洗脱);检测波长为254nm;流速为1mL/min;柱温为40℃。结果:10批双柏散中共标示出37个共有峰;全方特征峰基本为各药特征峰的加和,有22个峰归属大黄,6个峰归属关黄柏,4个峰归属侧柏叶,3个峰归属泽兰,2个峰归属薄荷,产生1个新特征峰。结论:采用对照品对照,鉴定了10个主要特征峰。更多还原

【Abstract】 AIM:To establish the method of fingerprint analysis on Shuangbai Powder(Radix et Rhizoma rhei,Cacumen platycladi,Cortex phellodendri amurensis,Herba lycopi and Herba menthae) to distinguish the characteristic fingerprint.METHODS:HPLC with ZORBAX Eclipse XDB-C18 was used,acetonitrile-0.1% H3PO4 solution(gradient elution) as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and column temperature was 40 ℃.RESULTS:Thirty-seven common peaks were separated from 10 batches of Shuangbai Powder.The characteristic peaks were the summation,22 peaks were from Radix et Rhizoma Rhei,6 peaks were from Cortex phellodendri Amurensis,4 peaks were from Cacumen Platycladi,3 peaks were from Herba Lycopi,and 2 peaks were from Herba Menthae,there was one new characteristic peak.CONCLUSION:Ten peaks gathered from Shangbai Powder consist of rhein,emodin,chrysophanol,aloe-emodin,physcion,gallic acid,berberine hydrochloride,palmatine;quercitrosid,and linarin.更多还原