【摘要】 目的 观察电针法对兔膝骨关节炎(KOA)模型治疗效果，探讨电针法治疗兔KOA的机制。方法 将18只健康新西兰兔随机分为空白对照组、模型对照组、电针法组，每组6只。空白对照组正常饲养，模型对照组、电针法组采用改良韦德曼左后肢伸直位固定制动法固定6周制作KOA模型，造模完成1周后给予电针法组电针治疗4周，每周5次，每次20 min。用奎森(Lequesne)功能指数评价兔左膝关节功能评分；酶联免疫吸附测定(ELISA)法检测血清及关节液白细胞介素(IL)-1β、IL-6水平；亚硝酸盐比色(Griess)法检测血清及关节液一氧化氮(NO)水平；苏木精-伊红(HE)染色法观察膝关节软骨组织形态学改变，曼金(Mankin)评分法评价软骨退变程度；免疫荧光技术观察膝关节软骨组织p-P38MAPK阳性细胞表达。结果 与空白对照组比较，模型对照组Lequesne评分增高(P<0.05);血清及关节液中IL-1β、IL-6、NO水平升高(P<0.05);软骨面欠光滑，Mankin评分升高(P<0.05);免疫荧光染色阳性细胞增多(P<0.05)。与模型对照组比较，电针法组Lequesne评分降低(P<0.05);血清及关节液中IL-1β、IL-6、NO水平降低(P<0.05);软骨面光滑，Mankin评分降低(P<0.05);免疫荧光染色阳性细胞减少(P<0.05)。结论 电针法能有效提高兔KOA模型膝关节行为能力，其机制可能是通过降低炎症因子表达水平，影响MAPK信号通路的表达，延缓KOA发病进程。更多还原

【Abstract】 Objective To observe the therapeutic effect of electroacupuncture on rabbit knee osteoarthritis(KOA) model and to explore the mechanism of electroacupuncture in the treatment of KOA in rabbits. Methods Eighteen healthy New Zealand rabbits were randomly divided into a blank control group, a model control group and an electroacupuncture group, with 6 rabbits in each group. The rabbits were kept normally in the blank control group, while the model control and electroacupuncture groups were fixed in a modified Videman left hind limb extension and braking position for 6 weeks to create a KOA model,and electroacupuncture was given to the electroacupuncture group 1 week after completion of the modeling for 4 weeks,five times a week for 20 min each time. The functional score of the left knee joint of rabbits was evaluated by Lequesne’s functional algorithm index; serum and joint fluid interleukin( IL)-1β and IL-6 levels were measured by enzyme-linked immunosorbent( ELISA) assay; serum and joint fluid nitric oxide( NO) levels were measured by Griess; morphological changes in knee cartilage were observed by hematoxylin-eosin( HE) staining,and the degree of cartilage degeneration was evaluated by Mankin’ s scale; p-P38MAPK positive cell expression in knee cartilage was observed by immunofluorescence technique. Results Compared with the blank control group,the Lequesne score increased in the model control group( P <0. 05); the levels of IL-1β,IL-6,and NO in serum and joint fluid increased( P < 0. 05); the cartilage surface was less smooth and the Mankin score increased( P < 0. 05); and the immunofluorescence staining positive cells increased( P < 0. 05). Compared with the model control group,the Lequesne score was reduced in the electroacupuncture group( P < 0. 05); the levels of IL-1β,IL-6,and NO in serum and joint fluid were reduced( P <0. 05); the cartilage surface was smoother,and the Mankin score was reduced( P < 0. 05); and the immunofluorescence staining positive cells were reduced( P < 0. 05). Conclusion Electroacupuncture can effectively improve knee joint behavior in rabbit KOA model,and the mechanism may be to delay the onset of KOA by decreasing the expression level of inflammatory factors and affecting the expression of MAPK signaling pathway.更多还原