【摘要】 为揭示NtMYB108转录因子在烟草中的功能，从栽培烟草K326的叶片腺毛中克隆到1条MYB基因的全长cDNA序列，命名为NtMYB108b。NtMYB108b的开放阅读框为828 bp，编码275个氨基酸。生物信息学预测显示，NtMYB108b蛋白定位于细胞核，属于R2R3-MYB型转录因子S20亚组成员，与绒毛状烟草亲缘关系最近，序列相似性为100.00%。实时荧光定量PCR分析显示，NtMYB108b在烟草腺毛中优势表达；NtMYB108b在团棵期叶片中相对表达量最高，在现蕾期5个叶位中的表达无显著差异。经乙烯利（ET）和水杨酸（SA）处理后，NtMYB108b的表达量显著上调，而经茉莉酸甲酯（MeJA）、赤霉素（GA）和脱落酸（ABA）处理后其表达量无显著变化；使用聚乙二醇（PEG）处理模拟干旱胁迫，处理2 d后NtMYB108b表达量上调，这说明NtMYB108b的功能可能涉及ET、SA信号转导和干旱胁迫。更多还原

【Abstract】 To clarify the functions of NtMYB108 transcription factors in tobacco, a full-length cDNA sequence of MYB gene from the glandular trichomes of tobacco cv. K326 was cloned and named as NtMYB108b. The open reading frame of NtMYB108b gene was 828 bp encoding 275 amino acids.Bioinformatic predictions showed that NtMYB108b protein was located in nucleus and was a member of the S20 subgroup of R2R3-type MYB transcription factor. NtMYB108b protein had the closest relationship with Nicotiana tomentosiformis, and the sequence similarity between them reached100.00%. Quantitative real-time PCR(qRT-PCR)analysis showed that NtMYB108b was predominantly expressed in tobacco glandular trichomes. NtMYB108b had the highest relative expression level in leaves at resettling stage. There was no significant difference in the expression levels of leaves at 5stalk positions during flower budding stage. After ethephon(ET) or salicylic acid(SA) treatment,the expression level of NtMYB108b was significantly up-regulated, however, there was no significant change after the treatments of methyl jasmonate(MeJA), gibberellin(GA), and abscisic acid(ABA).After polyethylene glycol(PEG) simulated drought stress, the expression level of NtMYB108b was up-regulated 2 days after the treatment, which indicated that NtMYB108b might involve in the ET, SA pathway and drought stress.更多还原