【摘要】 目的 分析m⁶A去甲基化酶FTO的抑制剂（FB23-2）对人胶质母细胞瘤干细胞活性的影响。方法 运用CCK-8法和神经球形成实验检测FB23-2和替莫唑胺对GSC的影响；体外有限稀释实验检测FB23-2对GSC自我更新的影响；EdU法检测FB23-2对GSC增殖的影响；流式细胞术检测FB23-2对GSC凋亡的影响。结果 CCK-8结果显示，FB23-2能够有效抑制GSC的细胞活性，其IC₅₀分别为7.11μmol·L^-1和4.63μmol·L^-1。成球实验证明，FB23-2处理组的GSC神经球的大小和数量较对照组明显减少。体外有限稀释实验表明，FB23-2可有效抑制GSC的自我更新能力；EdU参入实验显示，与对照组相比，处理组分别降至（70.59±13.74）%和（50.33±4.53）%。流式细胞术检测处理组细胞的凋亡率分别为（12.16±1.90）%和（16.77±1.17）%。结论 FTO抑制剂FB23-2可抑制GSC的生长和自我更新能力，靶向FTO可能是清除GSC的潜在策略之一。更多还原

【Abstract】 Aim To investigate the effect of m⁶A demethylase FTO inhibitor（FB23-2） on human glioblastoma stem cell activity. Methods The effects of FB23-2 and Temozolomide on GSC were detected by CCK-8 assay and neurosphere formation assay. The effect of FB23-2 on self-renewal of GSC was detected by limited dilution assay in vitro. The effect of FB23-2 on the proliferation of GSC was detected by EdU method. The effect of FB23-2 on apoptosis of glioblastoma stem cellswas detected by flow cytometry.Results CCK-8 as-say showed that FB23-2 could effectively inhibit thecell viability of GSC with IC₅₀ values of 7. 11 μmol·L^-1 and 4. 63 μmol·L^-1,respectively. The size andnumber of GSC neural sphere in FB23-2 treatmentgroup were significantly reduced compared with controlgroup.In vitrolimited dilution experiment showed thatFB23-2 effectively inhibited the self-renewal ability ofGSC. EdU incorporation experiment showed that com-pared with the control group,the treatment group decreased to（70. 59 ± 13. 74）% and（ 50. 33 ± 4.53）%,respectively. The apoptotic rates of the treatedgroup were（12. 16 ± 1. 90）% and（16. 77 ± 1. 17）%by flow cytometry.Conclusions FTO inhibitor FB23-2 can effectively inhibit GSC growth,self-renewal andthe formation of neural sphere. In addition,FB23-2 caninhibit the proliferation of GSC and induce its apoptosis.更多还原