【摘要】 目的探讨时间分辨荧光法(TRFIA)、化学发光免疫分析法(CLIA)和酶联免疫吸附法(ELISA)检测胃蛋白酶原Ⅰ(PGⅠ)的相关性,及其临床实用性。方法 在TRFIA法检测的临床标本中随机选择88份,分别用CLIA法和ELISA法再次检测PGⅠ,分析三种方法检测结果的相关性;对体检人群、消化科门诊患者和住院患者进行回顾性分析,验证TRFIA法检测PGⅠ对胃病筛查的可行性。结果 TRFIA法(Y)与CLIA法(X),ELISA法(X)PGⅠ检测结果均呈显著正相关(r分别为0.992 8和0.995 0,P<0.05),三种方法检测结果的相对偏差(SE%)均<10%;体检人群、消化科门诊患者、消化科住院患者的血清PGⅠ水平分别为156.0±63.8 ng/ml,197.8±113.8 ng/ml,269.5±156.5 ng/ml,三者差异具有统计学显著性意义(P<0.05)。结论 TRFIA法检测PGⅠ结果可靠、操作方便,未来可能用于胃病患者的筛查或辅助诊断。更多还原

【Abstract】 Objective To evaluate the correlation of three pepsinogen Ⅰ(PG I) detection methods and its clinical applications.Methods 88 serum samples were randomly selected,and PG Ⅰ was detected by using time-resolved fluoroimmunoassay(TRFIA),chemiluminescence immunoassay(CLIA) and enzyme-linked immunosorbent assay(ELISA).respectively.Then,their results were processed using regression and correlation analysis.To verify the feasibility of gastric disease screening by PG Ⅰ detection,3 575 people were divided into three groups gastroenterology outpatients,gastroenterology hospitalized patients,and physical examination population.Results It showed a significant positive correlation between any two of the three methods(P<0.05),and the detection values of the medical decision concentration by the three methods have good correlativity,and can be converted to each other.PG I serum levels were 156.0±63.8 ng/ml,197.8±113.8 ng/ml,and 269.5 +156.5 ng/ml in outpatients,hospitalized patients,and physical examination population,respectively.There was significantly different between the three groups(P<0.05).Conclusion PG I detection by TRFIA is simple and effective method.It may be used for the screening or auxiliary diagnosis of gastric disease in the future.更多还原