【摘要】 牛乳中含有3种主要的过敏原蛋白:酪蛋白、β-乳球蛋白和α-乳白蛋白。本文详细叙述沉淀法、离子交换层析法、凝胶过滤层析法、羟基磷灰石层析法、疏水相互作用层析法、高效液相色谱法以及膜技术等分离纯化技术在牛乳主要过敏原分离纯化中的研究进展。其中,离子交换层析与凝胶层析已被广泛使用,而沉淀法一般作为粗提纯过的步骤。羟基磷灰石层析与疏水相互作用层析法也较为常见,既可单独分离过敏原,又可与其他方法结合来分离过敏原。另外高效液相色谱法与膜技术则是进一步纯化的后续工作,以提高过敏原的纯度。更多还原

【Abstract】 The bovine milk contains three kinds of major allergen proteins such as casein, β-lactoglobulin and α-lactalbumin. This article has reviewed research progresses on purification methods of major milk allergens. The purification methods including precipitation, ion exchange chromatography, gel filtration chromatography, hydroxyapatite chromatography, hydrophobic interaction chromatography, high performance liquid chromatography and membrane technology have been discussed. Among them, ion exchange chromatography and gel filtration have been used widely, and precipitation method has been generally used in the rough purification step. Although hydroxyapatite chromatography and hydrophobic interaction chromatography are also common for the purification, they can not be used to isolate allergens individually and need to combine with other methods for the separation of allergens. High performance liquid chromatography and membrane technology are suitable for the follow-up purification to improve the purity of allergens.更多还原