【摘要】 建立了果实中花色素苷的HPLC测定方法,采用ODS-C18柱(250 nm×4.6 nm),流动相A:1.6%甲酸水溶液;B:1.6%甲酸甲醇溶液,梯度洗脱为0~5 min,85%A+15%B;5~10 min,80%A+20%B;10~30 min,72%A+28%B;30~36 min,40%A+60%B;36~48 min,100%B。检测波长510 nm,流速为1 mL/min。2种花色素苷在0.00104~0.0104 mg/mL范围内线性良好,最低检出限为0.02μg/mL,平均回收率为99.5%和99.25%,平均标准偏差为1.98%和2.50%,并测定了大石早生李和藤稔葡萄中花色素苷含量。更多还原

【Abstract】 The method of determining anthocyanin content in fruits by HPLC was established.A ODS-C18 column(250 nm×4.6 nm) was selected.The mobile phase A and B was water and methanol containing 1.6% formic acid respectively.The gradient elution was 0～5 min,85%A+15%B,5～10 min,80%A+20%B,10～30 min,72%A+28%B,30～36 min,40%A+60%B,and 36～48 min,100%B.The detection wavelength was 510 nm,and the flow rate 1 mL/min.The calibration curves for cyanin-3-glucoside and cyanin-3-rutiniside were linear within the range of 0.00104～0.0104 mg/mL.The mean recovery ratios for them were 99.5% and 99.25%,and the mean standard deviations for them 1.98% and 2.5%.The detection limit of this method was 0.02 μg/mL.The method was applied to Dashizaosheng plum and Fujiminori grape samples.更多还原