【摘要】 【目的】探讨针刺对急性实验性癫痫模型大鼠脑电功率和海马内氨基酸类神经递质含量的影响。【方法】SD大鼠40只随机分为正常组、模型组、针刺组和西药组;针刺组针刺大鼠大椎、丰隆、肝俞透胆俞,西药组以丙戊酸钠灌胃给药,均连续治疗3 d;除正常组外,其他3组均采用腹腔注射青霉素钠法复制急性癫痫大鼠模型,观察造模后70~85 min内顶中线Pz区脑电地形图各频段功率及大脑海马区中兴奋性氨基酸谷氨酸(Glu)、天门冬氨酸(ASP),抑制性氨基酸γ-氨基丁酸(GABA)、甘氨酸(Gly)、丙氨酸(Ala)、牛磺酸(Tau)含量的变化。【结果】模型组δ、θ、α1、α2、β1、β2各频段的功率均高于正常组(P<0.01),而针刺和丙戊酸钠均可显著性降低δ、θ、α1、α2、β1频段的功率(P<0.05或P<0.01);模型组海马区内兴奋性氨基酸Glu、ASP,抑制性氨基酸GABA、Ala、Gly、Tau的含量均高于正常组(P<0.01),而针刺和西药可降低模型大鼠异常升高的兴奋性氨基酸Glu、ASP及抑制性氨基酸Tau的含量(均P<0.05或P<0.01),针刺可升高抑制性氨基酸Ala的含量(P<0.01),但针刺对抑制性氨基酸GABA、Gly的含量及bGABA/bGlu比值无显著性影响。【结论】针刺可通过抑制痫性放电,调节海马内抑制性氨基酸与兴奋性氨基酸的水平而发挥抗癫痫作用。更多还原

【Abstract】 【Objective】To investigate the influence of acupuncture on the power of brain electrical activity mapping（BEAM） and hippocampal amino acid neurotransmitters in acute epilepsy rats.【Methods】Forty SD rats were randomized into groups A（normal）,B（model）,C（treated with acupuncture） and D（treated with sodium valproate）.Group C was pretreated with acupuncture on acupoints of Dazhui and Fenglong and with needling through Ganshu toward Danshu.Group D was pretreated with sodium valproate by gastric gavage.The treatment in groups C and D lasted 3 days.Except group A,the mice in other 3 groups received intraperitoneal injection of sodium penicillin to induce acute epilepsy.Within seventy to eighty-five minutes after modeling,different frequency-band power of BEAM in rat brain middle Pz region was detected,and rats hippocampal contents of excitatory amino acids such as glutamic acid（Glu） and aspartic acid（ASP） as well as inhibitory amino acids such as gamma-aminobutyric acid（GABA）,glycine（Gly）,alanine（Ala） and taurine（Tau） were examined.【Results】Power of frequency band δ,θ,α₁,α₂,β₁ and β₂ in group B increased（P<0.01 compared with group A）but that of frequency band δ,θ,α₁,α₂,β₁ decreased in groups C and D（P<0.05 or P<0.01 compared with group B）.Hippocampal contents of Glu,ASP,GABA,Ala,Gly and Tau in group B were higher than those in group A（P<0.01）,and Glu,ASP and Tau decreased in groups C and D（P<0.05 or P<0.01 compared with group B）,Ala increased in group C（P<0.01）.Contents of GABA,Ala and Gly as well as the ratio of GABA and Glu in group C did not differ from those in group B.【Conclusion】The anti-epilepsy mechanism of acupuncture may be related to the inhibition of epileptic discharge and to the regulation of contents of excitatory amino acids and inhibitory amino acids.更多还原