【摘要】 目的:越橘提取物具有抑制肿瘤生长的作用,但其对宫颈癌生长的影响鲜有报道。探讨越橘提取物对Hela细胞Bcl-2、Bax、Fas及FasL表达的影响。方法:用浓度为0、0.025、0.25、2.5和25μg/ml的越橘提取物处理Hela细胞24 h后,采用Hoechst33342/PI染色、DNA ladder法和Annexin V/PI双染观察越橘提取物对Hela细胞凋亡的诱导作用,并采用Western blot法检测Bcl-2、Bax、Fas及FasL蛋白表达。结果:0.25~25μg/ml的越橘提取物处理组细胞呈现明显的凋亡形态学改变和凋亡特异性条带,细胞凋亡率依次为(4.13±0.63)%、(5.41±0.77)%、(8.74±1.27)%和(12.05±1.03)%。同时Western blot结果显示越橘提取物处理组细胞中bax、Fas及FasL蛋白表达增加,而Bcl-2蛋白表达减少。结论:越橘提取物通过调节一些与凋亡相关蛋白的表达诱导Hela细胞的凋亡。更多还原

【Abstract】 Objective: Bilberry extract can inhibit the growth of cancer cells,but its effect on cervical carcinoma is rarely reported.This study was to explore the effects of bilberry extract on the expressions of Bcl-2,Bax,Fas and FasL in Hela cells.Methods: We determined the apoptosis of Hela cells by Hoechst33342/PI fluorescent staining and DNA ladder and Annexin V/PI double-staining after exposed to bilberry extract at the concentrations of 0.025-25 μg/ml for 24 hours,and the protein expressions of Bcl-2,Bax,Fas and FasL were detected by Western blotting.Results: Obvious apoptosis-associated morphological changes and specific DNA ladder bands were observed in the Hela cells after exposed to bilberry extract at the concentrations of 0.025,0.25,2.5 and 25 μg/ml,and the apoptosis rates of the cells were(4.13 ± 0.63)%,(5.41 ± 0.77)%,(8.74 ± 1.27)% and(12.05 ± 1.03)%,respectively.Western blot showed increased expressions of bax,Fas and FasL,and a decreased expression of bcl-2.Conclusion: Bilberry extract induces the apoptosis of Hela cells by up-regulating the protein expressions bax,Fas and FasL and down-regulating that of bcl-2.更多还原