【摘要】 目的　将构建的口腔黏膜异体移植修复腭裂术后遗留的裸露硬腭处 ,观察其对腭裂术后上颌骨继发畸形的防治效果。方法　分离提取SD乳鼠硬腭黏膜细胞并对其进行培养 ,以自制藻酸钠薄膜作为支架材料构建组织工程化的口腔黏膜。 3周龄雌性SD大鼠 ,随机分成 1个正常对照组和 3个实验组 ,每组 2 0只。正常对照组无任何处理 ,实验组均行左侧硬腭黏膜骨膜瓣切除 ,Ⅰ组切除后任其裸露 (裸露组 ) ,Ⅱ组、Ⅲ组分别行藻酸钠薄膜修复 (材料组 )和组织工程化的口腔黏膜修复 (黏膜组 )。术后 9周 (12周龄 )处死动物 ,体视显微镜下测量左右侧硬腭骨宽度 ,比较各组的非对称率。结果　裸露组与材料组左右侧硬腭骨宽度非对称率无差异 ;黏膜组非对称率小于裸露组和材料组。结论　组织工程化口腔黏膜移植修复裸露硬腭对腭裂术后上颌骨继发畸形有一定的预防效果。更多还原

【Abstract】 Objective To observe the effectiveness of prevention and cure for maxillary growth deformity following tissue engineered oral mucosa implantation on mucoperiosteal denuded palate process in young rat. Methods Hard palate mucoperiosteum of a SD baby rat were excised and oral keratinocytes were isolated and cultured. Tissue engineered oral mucosa was fabricated with the cultured oral keratinocytes and the membrane made of sodium alginate (SA). 80 female three-week-old SD rats were used as subjects in this study. The animals were divided randomly into a normal control group and 3 experimental groups, each group included 20 rats. Normal control group (NG) were not operated. Hard palate mucoperiosteum on left side in all experimental groups were excised, exposed bone were not treated in denuded group (DG), but repaired with membrane in material group (MG)and repaired with the tissue engineered oral mucosa in mucosal group (MUG). All the animals were sacrificed at 9th week postoperatively (12 weeks old), and the clean widths of right and left hard palatal were measured under a dissection microscope. The difference between palatal widths of two sides and the asymmetry ratio between the different groups were compared and analyzed.Results No significant difference in asymmetry was discovered between the DG and the MG, but the asymmetry in MUG was less than DG or MG.Conclusion Tissue engineered oral mucosal implantation in palatoplasty is an effective method in preventing and curing secondary maxilla deformity by repairing denuded bone wound.更多还原