【摘要】 采用表面活性剂十二烷基硫酸钠（SDS）协同超声波法提取泡桐花总黄酮，在单因素实验的基础上，采用响应面法优化泡桐花总黄酮的提取工艺，并评价其抗氧化活性。结果表明，泡桐花总黄酮的最佳提取工艺为：SDS质量分数0.55%、提取温度60℃、提取时间65 min、超声功率240 W,在此条件下，泡桐花总黄酮提取率为64.98 mg·g^-1,与预测值(65.03 mg·g^-1)接近；泡桐花总黄酮具有较强的DPPH自由基、ABTS自由基清除能力及铁还原力，对DPPH自由基、ABTS自由基的IC₅₀值分别为0.07 mg·mL^-1、0.10 mg·mL^-1。更多还原

【Abstract】 We extracted total flavonoids from Paulownia flowers by surfactant（sodium dodecyl sulfate, SDS）-ultrasonic method.Moreover, we optimized the extraction process of total flavonoids from Paulownia flowers by response surface methodologies on the basis of single-factor experiments, and evaluated its antioxidant activity.The results show that the optimal extraction conditions can be determined as follows: the SDS mass fraction of 0.55%,the extraction temperature of 60 ℃,the extraction time of 65 min, and the ultrasonic power of 240 W.Under above conditions, the extraction rate of total flavonoids from Paulownia flowers can reach 64.98 mg·g^-1,which is close to the predicted value(65.03 mg·g^-1).The total flavonoids from Paulownia flowers has strong scavenging ability to DPPH free radicals and ABTS free radicals and iron reducing ability, and the IC₅₀ values of total flavonoids from Paulownia flowers towards DPPH free radicals and ABTS free radicals are 0.07 mg·mL^-1 and 0.10 mg·mL^-1,respectively.更多还原