【摘要】 滑皮金柑(Fortunella crassifolia Swingle)为我国重要的柑橘类水果之一，为获得其完整的遗传信息，我们采用PacBio单细胞长链测序技术对滑皮金柑进行全长转录组测序，总获得13.81 G原始数据.经处理得到12 040个全长转录本，其中已知转录本为5 033个，已知基因的新转录本为5 589个，新基因转录本为1 418个，确定的新转录本占比为58%.全长转录本对应于6 984个基因，其中878个为新基因.经结构分析发现3 031个基因发生了可变剪切事件，占比为43.4%;6 106个基因至少含有一个poly(A)位点，且其中含有2个和2个以上的poly(A)位点的基因占比为42.3%;596个转录因子分布于82个转录因子家族；360个LncRNA序列和75个融合基因.通过对PacBio数据的进一步挖掘，该研究还发现了6个抗病基因，为滑皮金柑的抗病品种培育打下基础.更多还原

【Abstract】 Fortunella crassifolia Swingle is one of the most important citrus fruits crops in China. In order to obtain complete genetic information, single-molecule long read isoform sequencing(SMRT-seq) technology of PacBio was performed to sequence the full-length transcriptome of Huapi kumquat, and a total of 13.81 G raw data was produced. After analysis, we get 12,040 full-length transcripts including 5,033 known transcripts, 5,589 new transcripts of known genes, 1,418 new gene transcripts. 58% of transcripts were newly excavated transcripts that had not been previously annotated. 6,984 genes containing 878 new genes were annotated, and 3,031 genes(43.4%) were found to have alternative splicing events. 6,106 genes contained at least one poly(A) sites, and the proportion of genes containing two or more poly(A) sites was 42.3%. 596 transcription factors were identified and distributed in 82 transcription factor families. 360 LncRNAs and 75 fusion genes were identified. Beside, we also identified 6 resistance genes which will lay foundation for the breeding of disease-resistant varieties of Fortunella crassifolia Swingle in the future.更多还原