【摘要】 本试验旨在研究副干酪乳杆菌(Lactobacillus paracasei) KL1、枯草芽孢杆菌(Bacillus subtilis)Liu-c1微生态制剂对京红1号种母鸡生产性能、降血脂和抗氧化作用的影响。将384只健康、体重接近的54周龄京红1号种母鸡随机分成4组,每组6个重复,每个重复16只。空白组饲喂基础饲粮,试验组(KL1组、 Liu-c1组和复合组)分别在基础饲粮中添加8×107CFU/(只·d)的副干酪乳杆菌KL1、枯草芽孢杆菌Liu-c1和复合微生态制剂(副干酪乳杆菌KL1∶枯草芽孢杆菌Liu-c1=1∶1)。结果表明:1)与空白组相比,试验组产蛋率分别极显著增加5.77%、5.37%和8.84%(P<0.01),种蛋合格率分别极显著增加12.65%、9.15%和14.43%(P<0.01),料蛋比分别显著降低2.27%(P<0.05),极显著降低4.09%和5.45%(P<0.01);2)与空白组相比,试验组的红染脂肪滴皆有明显改善,粗脂肪含量显著降低28.43%、24.18%和27.08%(P<0.05);3)与空白组相比,KL1组血清、肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性、总抗氧化能力(T-AOC)和高密度脂蛋白(HDL)含量极显著提高(P<0.01),甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL)和丙二醛(MDA)含量极显著降低(P <0.01);4)与空白组相比,Liu-c1组血清和肝脏中TG含量极显著降低(P<0.01),SOD活性和HDL含量显著提高(P<0.05),TC、LDL和MDA含量显著降低(P<0.05),而GSH-Px活性和T-AOC有所增加(P>0.05);5)与空白组相比,复合组血清和肝脏中HDL含量极显著提高(P<0.01),MDA含量极显著降低(P<0.01),SOD、GSH-Px活性和T-AOC显著提高(P<0.05),TG、TC和LDL含量则显著降低(P<0.05)。综上所述,饲粮中添加枯草芽孢杆菌Liu-c1微生态制剂能够促进种母鸡消化吸收,提高生产性能;副干酪乳杆菌KL1具有降脂护肝、增强抗脂质过氧化能力;复合组微生态制剂兼顾提高种母鸡生产性能、抗氧化和降血脂能力,具有明显优势。更多还原

【Abstract】 This experiment was conducted to study the effects on microecological preparations of Lactobacillus paracasei KL1 and Bacillus subtilis Liu-c1 on performance,blood lipid capability and antioxidant of Jinghong No.1 breeding hens. A total of 384 healthy,weight nearly 54-week-old Jinghong No.1 breeding hens were divided into 4 groups with 6 replicates per group and 16 hens per replicate. Breeding hens in the blank control group were fed a basal diet,and the others in experimental groups were fed the basal diets supplement with 8×107 CFU/（hen·d）microecological preparations of Lactobacillus paracasei KL1,Bacillus subtilis Liu-c1 and their composition(Lactobacillus paracasei KL1 ∶ Bacillus subtilis Liu-c1 = 1 ∶ 1),respectively. The results showed as follows:1)compared with the blank control group,there was no significant difference in the average egg weight of the KL1 group,and the egg production rate was extremely increased by 5.77%,5.37% and8.84%(P< 0. 01),the qualified egg rate was extremely increased by 12. 65%,9. 15% and 14. 43%(P <0.01),the feed/egg was significantly reduced by 2.27%(P<0.05)and extremely reduced by 4.09% and5.45%(P<0.01). 2)Compared with the blank control group,red stained fat droplets in the KL1 group,Liuc1 group and composite group had improved significantly,and crude fat content significantly reduced by28.43%,24.18% and 27.08%(P<0.05). 3)In KL1 group,superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity,total antioxidant capacity(T-AOC)and high density lipoprotein(HDL)content in serum and liver were significantly increased(P<0.01),triglyceride(TG),total cholesterol(TC),low density lipoprotein(LDL)and malondialdehyde(MDA)contents were extremely decreased(P<0.01). 4)In Liu-c1 group,TG content in serum and liver was extremely decreased(P<0.01),SOD activity and HDL content were significantly increased(P<0.05),TC,LDL and MDA contents were significantly decreased(P<0.05),while GSH-Px activity and T-AOC were no significantly change(P>0.05). 5)In composite group,HDL content in serum and liver was extremely increased(P<0.01),MDA content was extremely decreased(P<0.01),SOD,GSH-Px activities and T-AOC were significantly increased(P<0.05),and TG,TC and LDL contents were significantly decreased(P<0.05). In conclusion,dietary microecological preparations supplementation,Bacillus subtilis Liu-c1 can promote the digestion and absorption and improve performance of breeding hens,Lactobacillus paracasei KL1 can reduce fat and protect the liver,inhibit the formation of fatty liver,reduce fat accumulation,and enhance the ability of anti-lipid peroxidation,the composite group has obvious advantages for improving the performance of breeding hens,anti-oxidation and lowering blood lipid.[Chinese Journal of Animal Nutrition,2021,33(10):5627-5636]更多还原