【摘要】 目的观察3,6-二羟基黄酮对乳腺癌细胞株MCF-7(雌激素受体阳性ER+)、MDA-MB-231(雌激素受体阴性ER-)、直肠癌细胞株LoVo、DLD-1和前列腺癌细胞株PC3增殖和凋亡的影响。方法MTT法检测3,6-二羟基黄酮对不同肿瘤细胞生长存活的影响,计算半数抑制浓度(IC50);DAPI染色观察3,6-二羟基黄酮对肿瘤细胞生长状态及形态的影响,Annexin V/PI双染流式细胞仪检测细胞凋亡变化。结果增殖检测结果表明,3,6-二羟基黄酮对于MCF-7、MDA-MB-231、LoVo、DLD-1和PC3细胞增殖均表现出显著抑制作用,48hIC50值分别为16.1、15.7、29.8、12.8和17.8μmol/L;细胞形态观察和凋亡检测结果表明,10μmol/L的3,6-二羟基黄酮作用24h,5种肿瘤细胞的生长状态和形态均发生显著变化,大量细胞表现出凋亡或死亡细胞的形态特征,细胞凋亡率均显著升高。结论3,6-二羟基黄酮具有显著的抗肿瘤效应,对于多种肿瘤细胞具有很强的增殖抑制和凋亡诱导作用。更多还原

【Abstract】 Objective To observe the effects of 3,6-dihydroxyflavone on the proliferation and apoptosis in breast cancer cell lines MCF-7 (ER+) and MDA-MB-231 (ER-),colorectal carcinoma cell lines LoVo and DLD-1,prostatic cancer cell line PC3. Methods Cell proliferation was detected by MTT assay. The morphological changes in cancer cells were observed after DAPI staining. Cell apoptosis was determined by flow cytometry after Annexin V/PI staining. Results MTT assay showed that 3,6-dihydroxyflavone could significantly inhibit the proliferation of MCF-7,MDA-MB-23,LoVo,DLD-1 and PC3 cell lines. The IC 50 value for 48 h was 16.1,15.7,29.8,12.8 and 17.8 μmol/L,respectively. Morphological observation and apoptosis detection indicated dramatic proapoptotic morphological changes and enhanced cell apoptosis rate in all 5 cancer cells after treatment with 3,6-dihydroxyflavone at the dose of 10 μmol/L for 24 h. Conclusion 3,6-dihydroxyflavone has potent anti-cancer activity and can significantly inhibit the proliferation and induce apoptosis in cancer cells.更多还原