【摘要】 目的　了解CTLA4Ig腺病毒在不同哺乳动物体内的转染能力及表达情况。方法　用所构建的CTLA4Ig腺病毒通过静脉或腹腔注射到Balb/c小鼠、Wistar大鼠、兔、小香猪、羊体内 ,应用蛋白质斑点印记检测CTLA4Ig在以上动物血清中的表达情况 ,同时还观察各动物脏器的病理改变及动物的生存情况。结果　重组腺病毒转染 7d后大鼠、小鼠血清中出现CTLA4Ig ,持续表达 2周。各动物未见异常死亡 ,各脏器未见明显病理改变。结论　CTLA4Ig重组腺病毒能够有效转染Balb/c小鼠、Wistar大鼠更多还原

【Abstract】 Objective To study CTLA4Ig expression in different mammalias.Methods The replication defective recombinant adenovirus of CTLA 4Ig was injected into Balb/c mouse?Wistar rat?Rabbite?Swine?Sheep. The expression of CTLA4Ig in serum was detected by Dot ELISA.Results The expression of CTLA 4Ig in serum could be detected in Balb/c mouse?Wistar rat at day 7 after injection.Conclusion The CTLA 4Ig gene could be transferred into Balb/c mouse?Wistar rat by intravenous and abdominal cavity administration.更多还原