【摘要】 目的:研究桃仁、木香、黄芪分别与红花配伍对实验性SD大鼠早期桡骨骨折愈合过程中骨痂厚度及BMP-2表达的影响,探讨骨折三期辨证的治疗方法。方法:256只SD大鼠造模后随机分为模型组、桃仁红花组、木香红花组、黄芪红花组4组,每组各64只,每组再随机平均分为4个小组,分别作为骨折后第3、7、10、14d组,每小组16只。于造模后第3、7、10、14d,处死1小组大鼠。取材进行常规HE染色观察骨痂厚度、BMP-2-DAB显色计数观察BMP-2阳性细胞数。结果:造模后第3d,各给药组与模型组比较,标本BMP-2阳性细胞数及骨痂厚度差异无统计学意义;造模后第7、10、14d,各给药组与模型组比较,标本BMP-2阳性细胞数及骨痂厚度差异有统计学意义(P<0.05,P<0.01),桃仁红花组、黄芪红花组与木香红花组比较,标本BMP-2阳性细胞数及骨痂厚度差异有统计学意义(P<0.05)。结论:木香与红花配伍能显著提高SD大鼠早期桡骨骨折断端局部BMP-2的表达水平,增加骨痂厚度,就促进SD大鼠骨折愈合过程中BMP-2表达作用而言,理气活血法可能是四肢闭合骨折早期的较佳治法。更多还原

【Abstract】 Objective: To observe the effect of different compatibility of herbs on BMP-2 expression at fracture site and thickness of callus of early radius fracture rat models. Methods: 256 SD rats were divided into four groups randomly: model group, Semen Persicae and Flos Carthami group, Radix Aucklandiae and Flos Carthami group, Radix Astragali and Flos Carthami group. Left radius fracture rat models were established. Conventional haematoxylin and eosin staining and immunostaining for bone section were carried out on days 3,7,10 and 14 after model establishment. Results: On days 7, 10 and 14 after model establishment, compared with model group, BMP-2 positive cells at fracture site and thickness of callus were significantly increased in all the treatment groups; compared with Radix Aucklandiae and Flos Carthami group, BMP-2 positive cells at fracture site and thickness of callus were significantly decreased in Semen Persicae and Flos Carthami group, Radix Astragali and Flos Carthami group. Conclusions: Radix Aueklandiae and Flos Carthami can increase obviously BMP-2 expression at fracture site and thickness of callus in the healing of early radius fracture rats. We can get a conclusion that the therapy of regulating the flow of qi and promoting the circulation of blood was an effective therapy on early extremities closed fracture from this experiment.更多还原